Inositol hexakisphosphate synthesis in plant suspension-cultured cells and measurement of inositol phosphate isomers with anion chromatography
نویسندگان
چکیده
1 Department of Biology, Faculty of Science, Kobe University, Rokkodai 1-1, Nada-ku, Kobe, 657-8501, Japan 2 Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chuou-ku, Tokyo, 113-0027, Japan 3 Department of Chemistry, Pohang University of Science and Technology, San 31 Hyoja Dong, Pohang 790-784, Korea 4 Department of Life Science, Graduated School of Science, University of Hyogo, Harima Science Garden City, Hyogo 678-1297, Japan
منابع مشابه
Separation of phytic acid and other related inositol phosphates by high-performance ion chromatography and its applications.
A high-performance anion-exchange chromatographic method was developed for the separation of phytic acid and other inositol phosphates (myo-inositol bis-, tris-, tetrakis-, and pentakisphosphates) with gradient elution and ultraviolet absorbance detection after post-column derivatization. With the acidic eluents, the combination of anion-exchange and ion suppression retention mechanisms led to ...
متن کاملTurnover of inositol pentakisphosphates, inositol hexakisphosphate and diphosphoinositol polyphosphates in primary cultured hepatocytes.
We have used a non-transformed cell model, the primary cultured hepatocyte, to explore the turnover of inositol hexakisphosphate, multiple isomers of inositol pentakisphosphate and two novel diphosphoinositol polyphosphates. All of these compounds gradually accumulated radioactivity throughout a 70 h period of labelling with [3H]inositol. However, a rapid metabolic rate was revealed upon inhibi...
متن کاملSynthesis of inositol phosphate ligands of plant hormone-receptor complexes: pathways of inositol hexakisphosphate turnover.
Reduction of phytate is a major goal of plant breeding programs to improve the nutritional quality of crops. Remarkably, except for the storage organs of crops such as barley, maize and soybean, we know little of the stereoisomeric composition of inositol phosphates in plant tissues. To investigate the metabolic origins of higher inositol phosphates in photosynthetic tissues, we have radiolabel...
متن کاملPurification of myo-Inositol 1-Phosphate Synthase from Rice Cell Culture by Affinity Chromatography.
myo-Inositol 1-phosphate synthase (EC 5.5.1.4) is the enzyme which catalyzes the synthesis of the precursor for the myo-inositol oxidation pathway. Rice callus grown in suspension culture provides a good source of plant enzyme. Use has been made of a noncompetitive inhibitor to prepare an affinity column for this enzyme. With this column, the enzyme from rice callus has been purified 1500-fold ...
متن کاملPhytate (Inositol Hexakisphosphate) in Soil and Phosphate Acquisition from Inositol Phosphates by Higher Plants. A Review
Phosphate (P) fixation to the soil solid phase is considered to be important for P availability and is often attributed to the strong binding of orthophosphate anion species. However, the fixation and subsequent immobilization of inositolhexa and pentaphosphate isomers (phytate) in soil is often much stronger than that of the orthosphate anion species. The result is that phytate is a main organ...
متن کامل